These results suggest that a antioxidant-grafting membrane could offer protection for patients against oxidative stress and would be helpful for reducing the dosage of anticoagulant during hemodialysis. The effects of antioxidants grafting on the blood coagulation, platelet aggregation, and oxidative stress were evaluated using human blood. The complete blood count (CBC) and coagulation time (CT) was evaluated in vitro for the hemocompatibility. In vitro evaluation was carried out using mini- hemodialyzer circulating with fresh porcine whole blood to simulating kidney therapy. The dialysis performance and hemocompatibility were estimated. In this study, hollow fiber membrane (PAN,CA and PSF) was hydrolyzed and grafted with antioxidants such as conjugated linoleic acid (CLA)or heparin. Furthermore, the removal of bacterial endotoxin (lipopolysaccharide, LPS) by antioxidant-bonding membrane was measured with ELISA. In addition, the production of reactive oxygen species (ROS) was measured by chemiluminescence (CL) method to evaluate the oxidative stress.
Effect of antioxidants grafting on the hemocompatibility of HD. Antioxidants grafting on the hemocompatibility of hemodialyzer membranes
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