This work details a strategy that optimizes yield and purity without jeopardizing the integrity of the fusion protein. Therefore, a purification strategy was developed taking advantage of this commonly used reporter molecule. Reporter molecules, such as green fluorescent protein, are often used to monitor recombinant protein expression levels. Issues include high levels of protease activity, tannin and phenolic reactivity and hydrophobic aggregation. Purifying recombinant proteins from plants is often met with difficulties due to the complexity of plant cell extracts. Although previous methods have been developed for purifying GFP, these strategies often included exposure to protein-denaturing solvents or required gel filtration and protein concentrating steps. Difficulties in extraction and purification are major hurdles preventing the routine use of plants and plant suspension cells as bioreactors.
A Green Fluorescent Protein-Based Purification System for Plant Cells. Optimizing Yield and Recovery of Recombinant Proteins from Plants
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Книга по Требованию
Дослідження механізму дії гіпервисокочастотного випромінювання на загоювання шкірних ран в...
УКРАЇНСЬКИЙ НАУКОВО-ДОСЛІДНИЙ ІНСТИТУТ МЕДИЧНОЇ РЕАБІЛІТАЦІЇ ТА КУРОРТОЛОГІЇ МОЗ УКРАЇНИ ШЕВЦОВ БОГДАН МИКОЛАЙОВИЧ УДК 615.849.11:616.5-001.41-002.3-089 ДОСЛІДЖЕННЯ МЕХАНІЗМУ ДІЇ ГІПЕРВИСОКОЧАСТОТНОГО ВИПРОМІНЮВАННЯ НА ЗАГОЮВАННЯ ШКІРНИХ РАН В ЕКСПЕРИМЕНТІ 14.01.33 – Медична реабілітація, фізіотерапія та курортологія АВТОРЕФЕРАТ дисертації на здобуття наукового ступеня кандидата медичних наук ОДЕСА – 2008 Дисертацією є рукопис. Робота виконана у відділі консервативного лікування та реабілітації Державної установи s mecha ic i juries was es ablished. he favorable dose-depe de ac io of HHF radia io o wou d exsuda e cell co e a d o cellular immu oreac ivi y markers duri g wou d purule complica io s was proved. he op imal parame ers of HHF radia io i flue ce o mecha ic ski wou d heali g are defi ed. hese parame ers are laid i base of worki g ou of physio herapeu ic me hodic of o e-i fec ive a d i fec ive ski wou ds rea me . Key words: radia io , hyperhigh-freque cy, irradia io , ski wou ds, heali g. ПЕРЕЛІК УМОВНИХ ПОЗНАЧЕНЬ, СКОРОЧЕНЬ ГВЧ- гіпервисокі частоти ЕМВ- електромагнітне випромінювання КВЧ- крайньовисокі частоти КУО – колонієутворююча одиниця HC – воднево-цианіста газова суміш (діюча речовина лазера)
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