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RNA interference Screens as a Tool for Discovering Gene Function. for cancer genetics studies

Формат:      Страниц 208
     мягкая обложка
Despina S.    
Книга по Требованию    
3051 руб
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Recent advances in the field of RNA interference (RNAi) have enabled researchers to conduct in depth investigations of gene function using RNAi libraries. Each hairpin is linked to a unique 60 nucleotide identification sequence, which serves as a barcode and allows us to virtually count the number of cells that contain a specific hairpin in a cell population. The barcode can be amplified from a cell' s genomic DNA and fluorescently labeled to produce a probe that is hybridized to a microarray. I applied this RNAi barcode screening method in a screen using a complex mixture of 7500 library hairpins in a p53 isogenic HCT 116 colon cancer cells to identify genes that modify sensitivity to a common chemotherapeutic, doxorubicin. This work illustrates the powerful use of RNAi screens to search for genes that synergize with existing therapeutics, and suggests strategies for genetically informed combination therapies. Our second-generation shRNA libraries consist of over 200,000 short hairpin RNA constructs targeting over 45,000 human and mouse genes modeled after primary miRNA transcripts. Small changes in barcode copy number can be monitored through the use of microarray technology.
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Организация корпоративной компьютерной сети в...
Когда новый компьютер подключается к сети, обслуживаемой сервером DHCP, o запрашивает уни­кальный IP-адрес, а сервер DHCP назначает его из пула доступных адресов, Этот процесс состоит из четырех шагов: 1. Клиент DHCP запраши­вает IP-адрес (DHCP Discover, обнаружение), 2. DHCP-сервер предлагает адрес (DHCP Offer, предложение), 3. Клиент принимает предложение и запрашивает адрес (DHCP Reques , запрос) и адрес официально назначается сервером (DHCP Ack owledgeme , подтверждение). Чтобы адрес не ПРИЛОЖЕНИЕ 1 Панель инструментов администратора сети ПРИЛОЖЕНИЕ 2 Панель службы DHCP ПРИЛОЖЕНИЕ 3 Панель службы D S ПРИЛОЖЕНИЕ 4 Панель настройки свойств I ER E ПРИЛОЖЕНИЕ 6 Панель настройки свойств локальной сети ПРИЛОЖЕНИЕ 7 Вид консоли управления сервером

Effect of RNA interference and Microarray. Expression level of snoRNAs Книга по Требованию Muhammad N.A.
Very little literature exists regarding RNAi against non-coding RNA (hot stuff nowadays) and the expression profile of non-coding RNAs.
2416 руб
Domain Driven Program Evolution. Run time code generation and inspection as tool for high level optimisations Книга по Требованию Diego C.
In this work we investigate a particular class of programs capable of evolving their own structure over time in order to adapt to particular execution conditions and to the user.
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The issue of HIV/AIDS is a current area of discourse .
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STED microscopy as a tool in cell biology. Investigation of age-related alterations occurring on the protein and the lipid level by using STED microscopy Книга по Требованию Rebecca M.
Furthermore this technique allowed the detection of early alterations in these patterns in dependence of the provided glucose amount.
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Home Drug Screening - A Parent's Ally in the War on Drugs. An Assessment of Home Drug-Test Kits as a Deterrent to Adolescent Drug Use Книга по Требованию
The intent of this study, was to examine the effects of home drug-test kits as a deterrent to adolescent drug use.
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Co-Branding as a Tool for Strategic Brand Activation. How to Find the Ideal Partner - An Explanatory Case Study in the Fashion and Design Sector Книга по Требованию Natalia D.
Natalia Dorozala and Antonia Kohlbrenner show the importance of partner selection in the context of co-branding and explain that partner selection depends decisively on the different co-branding aims and incorporates the crucial step when using co-branding as a tool for strategic brand activation.
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Storytelling as a PR Tool in the Tourism Industry. A Situational Analysis of the Application and Importance of PR and Storytelling Книга по Требованию Karin M.
The tourism industry can be characterized based on its size and intangibility of services and products offered.
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Successful Two-Way Communication Online Communities as a Public Relations Tool. Книга по Требованию Valerie P.
One tool to encourage and allow open discussion channels on the internet are online communities.
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Classifying Phone Usage Data. Usage of Empirical Distribution Functions as Basic Variable for Cluster Analysis to Characterize the Behavior of Telephone Subscribers Книга по Требованию
The use of the method is clearly exemplified within the scope of the KDD process (knowledge discovery in databases).
3234 руб
Knowledge as an effective tool to improve Economic Performance. Study in Micro and Small Enterprise Книга по Требованию Boris H.
To Understand and Explain about the effect of creation, the use and management of knowledge as a factor of economic performance in the micro and small business, utilizing a qualitative approximation based on the use of narratives, is the purpose of this study, for which a scheme based in a theoretical analysis in order to understand the behavior of the micro and small enterprises, related to the economic performance, the complexity of these sector and the public polices to develop for help them, on the other hand review all the theoretical contribution in knowledge and knowledge management and define what are the available tools, in the field of knowledge, to help to increase the economic performance of this particular enterprises.
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A Study of the Big Five Traits Personality and Leadership as Dispatcher Retention Tools. Книга по Требованию Lora R.
The study evaluates linkages between individual characteristics, contextual performance, servant leadership, and person-job fit, as related to dispatcher retention.
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For Whose Benefit? . An Investigation Into the Limits of Fair Trade as a Development Tool and the Risk of Clean-Washing The Economics of Fair Trade Coffee: Книга по Требованию Pierre K.
Finally, an original empirical strategy is proposed to see whether consumer awareness is making any difference and/or if supermarkets are clean-washing their reputation in the fair trade business.
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New Horizons in the Field of Management Audit:. As a Tool for Improving the Overall Management of Enterprises A Guideline for Good Governance Книга по Требованию Mohammed S.H.
Recently few firms have attempted to measure and assess the new intangible knowledge.
1923 руб
From systems design to randomised controlled trial Telerehabilitation as a clinical tool for Physiotherapists. Книга по Требованию Trevor R.
This work outlines the development of a telemedicine system to meet the unique needs of clinical physiotherapists and their patients.
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A Numerical-Analytical Method for Particle Accelerators. A Reworking Mode Matching Technique as a Testing Tool for Analytical and Experimental Methods in Accelerators Physics Книга по Требованию Marco P.
The Coupling Impedance is one of the most important parameters in Particle Accelerator Design.
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Urban Design and Users' Preferences. Virtual Reality Simulation as a Tool to Test Users' Preferences for Urban Design Alternatives Книга по Требованию Sherif E.
The book should be especially useful to students of architectural design and urban planning, or researchers in the fields of man-environment relationship and urban design simulation.
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repetition and quotation. as a tool for analyzing contemporary art Книга по Требованию agnieszka r.
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La Questione Meridionale. Social capital as a tool to understand the development disparity between the North and the South of Italy Книга по Требованию Gabriele D.T.
The fact that despite a huge amount of subsidies the South has not been able to reduce the development gap with the North has urged scholars to focus their attention on the capability of the society to improve its own situation that means to take into consideration sociological aspects with respect to economic development.
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The Fundamentals: Видеокурс: 2 видеокассеты - - с. English as a second language American business English ESL: Advanced English: Delta Systems /Афон-Паблишинг
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Bryan Ferry 'As Time Goes By' [CD] { } ~54.00.00 14256 Бомба-М
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Молочный гриб можно использовать для похудения, восстановления микрофлоры, очищения организмаМолочный гриб можно использовать для похудения, восстановления микрофлоры, очищения организма

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