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RNA interference Screens as a Tool for Discovering Gene Function. for cancer genetics studies

Формат:      Страниц 208
     мягкая обложка
Despina S.    
Книга по Требованию    
3051 руб
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Recent advances in the field of RNA interference (RNAi) have enabled researchers to conduct in depth investigations of gene function using RNAi libraries. Each hairpin is linked to a unique 60 nucleotide identification sequence, which serves as a barcode and allows us to virtually count the number of cells that contain a specific hairpin in a cell population. The barcode can be amplified from a cell' s genomic DNA and fluorescently labeled to produce a probe that is hybridized to a microarray. I applied this RNAi barcode screening method in a screen using a complex mixture of 7500 library hairpins in a p53 isogenic HCT 116 colon cancer cells to identify genes that modify sensitivity to a common chemotherapeutic, doxorubicin. This work illustrates the powerful use of RNAi screens to search for genes that synergize with existing therapeutics, and suggests strategies for genetically informed combination therapies. Our second-generation shRNA libraries consist of over 200,000 short hairpin RNA constructs targeting over 45,000 human and mouse genes modeled after primary miRNA transcripts. Small changes in barcode copy number can be monitored through the use of microarray technology.
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Организация корпоративной компьютерной сети в...
Когда новый компьютер подключается к сети, обслуживаемой сервером DHCP, o запрашивает уни­кальный IP-адрес, а сервер DHCP назначает его из пула доступных адресов, Этот процесс состоит из четырех шагов: 1. Клиент DHCP запраши­вает IP-адрес (DHCP Discover, обнаружение), 2. DHCP-сервер предлагает адрес (DHCP Offer, предложение), 3. Клиент принимает предложение и запрашивает адрес (DHCP Reques , запрос) и адрес официально назначается сервером (DHCP Ack owledgeme , подтверждение). Чтобы адрес не ПРИЛОЖЕНИЕ 1 Панель инструментов администратора сети ПРИЛОЖЕНИЕ 2 Панель службы DHCP ПРИЛОЖЕНИЕ 3 Панель службы D S ПРИЛОЖЕНИЕ 4 Панель настройки свойств I ER E ПРИЛОЖЕНИЕ 6 Панель настройки свойств локальной сети ПРИЛОЖЕНИЕ 7 Вид консоли управления сервером

Effect of RNA interference and Microarray. Expression level of snoRNAs Книга по Требованию Muhammad N.A.
Very little literature exists regarding RNAi against non-coding RNA (hot stuff nowadays) and the expression profile of non-coding RNAs.
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In this work we investigate a particular class of programs capable of evolving their own structure over time in order to adapt to particular execution conditions and to the user.
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The intent of this study, was to examine the effects of home drug-test kits as a deterrent to adolescent drug use.
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One tool to encourage and allow open discussion channels on the internet are online communities.
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The use of the method is clearly exemplified within the scope of the KDD process (knowledge discovery in databases).
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To Understand and Explain about the effect of creation, the use and management of knowledge as a factor of economic performance in the micro and small business, utilizing a qualitative approximation based on the use of narratives, is the purpose of this study, for which a scheme based in a theoretical analysis in order to understand the behavior of the micro and small enterprises, related to the economic performance, the complexity of these sector and the public polices to develop for help them, on the other hand review all the theoretical contribution in knowledge and knowledge management and define what are the available tools, in the field of knowledge, to help to increase the economic performance of this particular enterprises.
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A Study of the Big Five Traits Personality and Leadership as Dispatcher Retention Tools. Книга по Требованию Lora R.
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For Whose Benefit? . An Investigation Into the Limits of Fair Trade as a Development Tool and the Risk of Clean-Washing The Economics of Fair Trade Coffee: Книга по Требованию Pierre K.
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New Horizons in the Field of Management Audit:. As a Tool for Improving the Overall Management of Enterprises A Guideline for Good Governance Книга по Требованию Mohammed S.H.
Recently few firms have attempted to measure and assess the new intangible knowledge.
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